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ORIGINAL ARTICLES
Comparison of antimicrobial efficacy of propolis,
Morinda citrifolia
,
Azadirachta indica
, triphala, green tea polyphenols and 5.25% sodium hypochlorite against
Enterococcus fecalis
biofilm
Paridhi Garg, Shashi Prabha Tyagi, Dakshita Joy Sinha, Udai Pratap Singh, Vibha Malik, Edgar Richard Maccune
September-December 2014, 4(3):122-127
DOI
:10.4103/1658-5984.138141
Introduction:
Endodontic infections are polymicrobial in nature.
Enterococcus fecalis
is the most common micro-organism isolated from failed endodontic cases. The constant increase in antibiotic resistant strains and side effects caused by synthetic drugs has prompted researchers to look for herbal alternatives since the gold standard for irrigation i.e., sodium hypochlorite (NaOCl) has many disadvantages.
Objective:
The present study was aimed to explore newer irrigation solutions, which would probably be as effective as NaOCl.
Materials and Methods:
Extracted human single rooted premolar teeth were biomechanically prepared, vertically sectioned, placed in tissue culture wells exposing the root canal surface to
E. fecalis is
grown on Mueller Hinton agar plates to form a biofilm for 6 weeks. At the end of 6
th
week, all seven groups were treated with 3 ml of test solutions and control for 10 minutes and evaluated for
E. fecalis
growth and number of colony forming units.
Results:
Propolis, NaOCl and triphala showed no statistically significant difference, whereas all the other inter-group differences were statistically significant (Tukey's honest significant difference (HSD)) (
P
< 0.001).
Conclusion:
Propolis and triphala were found to be as efficacious as NaOCl. The use of herbal alternatives as root canal irrigation solutions might prove to be advantageous considering several unfavorable properties of NaOCl.
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7,647
1,371
REVIEW ARTICLES
Endodontic disinfection: Tsunami irrigation
Clifford J Ruddle
January-April 2015, 5(1):1-12
DOI
:10.4103/1658-5984.149080
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10,783
1,880
ORIGINAL ARTICLES
Antibacterial effect of silver nanoparticles against
Enterococcus faecalis
Zainab Ali Alabdulmohsen, Abdelhamied Yousef Saad
January-April 2017, 7(1):29-35
DOI
:10.4103/1658-5984.197989
Objective:
This study aimed to investigate the bactericidal effect of silver nanoparticles (AgNPs) in reducing bacterial infection in root canal when used as intracanal medicament alone or in addition to the conventionally used calcium hydroxide (Ca(OH)
2
).
Materials and Methods:
The root canals of 110 single-rooted teeth were cleaned, shaped, and sterilized. All groups, except for negative control, were inoculated with
Enterococcus faecalis
for 48 h. Then, teeth were divided into five groups according to the intracanal medicaments to be used. Group I (
n
= 30): Ca(OH)
2
. Group II (
n
= 30): AgNP. Group III (
n
= 30): AgNP + Ca(OH)
2
. Group IV (
n
= 10) was used as a positive control where the root canals were inoculated with
E. faecalis
and left without treatment. Group V (
n
= 10) was used as a negative control where the root canals were checked for the absence of bacterial growth. Specimens were incubated for 1 and 2 weeks. Pre- and post-medication samples were obtained by paper points, and the colony-forming units were counted.
Results:
Ca(OH)
2
resulted in a higher percentage of bacterial reduction in both 1 and 2 weeks of application (81.5% and 98%, respectively). AgNP was ineffective against
E. faecalis
with 32.9% bacterial reduction in 1 week and 56.5% after 2 weeks.
Conclusion:
It was concluded that the antibacterial effect of AgNP was lower than Ca(OH)
2
or combination of both materials.
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3,802
668
Radiographic assessment of quality of root fillings performed by undergraduate students in a Malaysian Dental School
Pishipati Vinayak Kalyan Chakravarthy, Jeyavel Rajan Karuna Moorthy
May-August 2013, 3(2):77-81
DOI
:10.4103/1658-5984.118155
Objective:
The aim of the study was to assess the radiographic quality of root fillings done by the undergraduate students.
Materials and Methods:
A sample of 258 clinical cases of patients who received root canal treatment from undergraduate students in the years 2010 to 2012 were selected, out of which 194 root fillings were assessed radiographically based on the following criteria; length and density of root filling. Root fillings were categorized as acceptable and unacceptable. Percentages and
χ
² test were used for statistical analysis.
Results:
From the 194 root fillings, 119 (61.35%) were having acceptable and 75 (38.65%) were having unacceptable root fillings. From the total 75 unacceptable root fillings, 49 (65.33%) had density problems, 11 (14.66%) were overfilled, and 15 (20%) were under filled. Chi-square test was done for statistical analysis; revealed no significant difference in the radiographic quality between anterior and posterior root fillings.
Conclusion:
The radiographic quality of root fillings done by undergraduate students was acceptable in 61.35% cases. Most common cause of unacceptable fillings was due to density problems compared to overfilling and under filling. Results from the study indicate that remedial measures have to be undertaken to improve training of undergraduate students in root canal treatment procedure.
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3,795
528
Radiographic investigation of
in vivo
endodontically treated maxillary premolars in a Saudi Arabian sub-population
Saad Al-Nazhan, Abdullah Al-Daafas, Nassr Al-Maflehi
January-April 2012, 2(1):1-5
DOI
:10.4103/1658-5984.104407
Aim:
To determine the prevalence of the number of root canals in permanent maxillary first and second premolars of a Saudi Arabian sub-population. Results will be compared to previous Asian studies.
Materials and Methods:
A total of 894 periapical radiographs of endodontically treated maxillary first and second premolars of 628 Saudi patients (268 males and 360 females) were viewed. The teeth were segregated into maxillary first premolars (463) and maxillary second premolars (431). The diagnostic, working length, master cone and final films with different angles were mounted, projected and, with the utilization of written clinical records, evaluated. Teeth with multiple canal systems were categorized according to whether the canals exited the root by common or separate apical foramen. Data was analyzed statistically using Chi-square test and professional
t
-test, by comparing pairs of groups with the significant level established at 5% (
P
< 0.05).
Results:
More than 90% of first maxillary premolar and more than 50% of the second maxillary premolar was found to have two canals. There was no significant difference between male (92%) and female (95%) in the distribution of the two root canals of the first maxillary premolar (
t
-test = 1.21,
P
value = 0.228), however, there was significant difference between the distributions of male (69.4%) and female (52.2%) of the two root canals within the second maxillary premolar (
t
-test = 3.75,
P
value = 0.000). Few teeth showed three canals.
Conclusion:
The number of root canals of the maxillary first premolar in Saudi population shows a higher incidence of two canals (93.6%) than previously reported. In addition, the figure is higher than most of the Asian countries.
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4
3,841
708
Adhesion to pulp chamber dentin: Effect of ethanol-wet bonding technique and proanthocyanidins application
Pallavi Sharma, Rajni Nagpal, Shashi Prabha Tyagi, Udai Pratap Singh, Naveen Manuja
January-April 2015, 5(1):38-45
DOI
:10.4103/1658-5984.149086
Aim:
To evaluate the microleakage of a simplified etch-and-rinse adhesive bonded to pulp chamber dentin with water-wet bonding (WWB) or ethanol-wet bonding (EWB) with and without proanthocyanidins (PA) application.
Materials and Methods:
Total 88 non-carious extracted human molar teeth were sectioned horizontally to expose the pulp chambers 1.5 mm coronal to the cemento-enamel junction. After the pulp tissue extirpation, canal orifices were enlarged and the root ends were sealed. The samples were randomly divided equally into following four groups according to the four bonding techniques performed using Adper Single Bond 2 [SB] adhesive (1) WWB; (2) EWB; (3) WWB and PA application [WWB + PA]; (4) EWB and PA application [EWB + PA]. Composite resin restorations were performed in all the pulp chambers. Total 20 samples from each group were subjected to microleakage evaluation, and two samples per group were assessed under scanning electron microscope for interfacial micromorphology.
Results:
The least microleakage score was observed in group 2 (EWB) with similar results seen in group 4 (EWB + PA) (
P
= 0.918). Group 2 (EWB) showed significantly less microleakage than group 1 (WWB;
P
= 0.002) and group 3 (WWB + PA;
P
= 0.009). Group 4 (EWB + PA) also depicted significantly reduced microleakage as compared with group 1 (WWB;
P
= 0.001) and group 3 (WWB + PA;
P
= 0.003).
Conclusion:
The use of EWB technique in a clinically relevant simplified dehydration protocol significantly reduced microleakage in simplified etch-and-rinse adhesive, Adper Single Bond 2, bonded to pulp chamber dentin. Application of PA had no significant effect on the microleakage of the adhesive bonded with either WWB or EWB.
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3,352
447
Knowledge regarding technical aspects of non-surgical root canal treatment in Al-Madinah Al-Munawarah private dental centers
Mothanna Alrahabi, Mohmmad Sami Ahmad
September-December 2015, 5(3):155-160
DOI
:10.4103/1658-5984.163625
Objective:
We evaluated knowledge of technical aspects of non-surgical root canal treatment among general dentists in private dental clinics in Al-Madinah Al-Munawarah, Saudi Arabia.
Materials and Methods:
A questionnaire was distributed randomly to 70 general dental practitioners who were working in private dental centers. The response rate was 88.6%.
Results:
The results showed that only 3% of dentists were using rubber dams for isolation during endodontic treatment. The majority (59.7%) were using radiographs to determine the length. Also, 37% were using nickel-titanium (NiTi) rotary instruments to prepare the root canal. The lateral condensation technique with gutta percha points to fill the root canal was used by most respondents (77.5%).
Conclusions:
This study provides important data on endodontic treatment by general dental practitioners in Al-Madinah Al-Munawarah private dental centers. It shows a general lack of knowledge regarding the importance of using rubber dams as well as the new endodontic materials and methods. Continuing education programs to update their knowledge in the field of endodontics are essential.
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2,665
4,179
REVIEW ARTICLES
Root and root canal morphology of Saudi Arabian permanent dentition
Ibrahim Ali Ahmad
May-August 2015, 5(2):99-106
DOI
:10.4103/1658-5984.155446
The aims of this paper were to review the studies that investigated the root and root canal morphology of permanent dentition in the Saudi Arabian population and to compare their results with the findings of international morphological studies conducted on other populations. An electronic search using the PubMed and Scopus databases was conducted using combination of the following keywords: "root canal morphology", "root morphology", "dental anomalies", and "Saudi Arabian population". In addition, all issues of the Saudi Dental Journal (since 1989) and Saudi Endodontic Journal (since 2011) were manually searched for relevant articles. Then, the reference lists of the articles were screened to identify further eligible publications. A total of 23 studies met the inclusion criteria and were subjected to data extraction and analysis. Variations from the normal root and canal morphology may occur in all populations including the Saudi Arabian population. Therefore, the clinicians must always take these variations in consideration during root canal treatment to ensure a successful treatment outcome.
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6,930
848
The anatomy of the root apex: A review and clinical considerations in endodontics
Osama S Alothmani, Nicholas P Chandler, Lara T Friedlander
January-April 2013, 3(1):1-9
DOI
:10.4103/1658-5984.116273
Studies on the anatomy of the root apex are an area of interest to the endodontist; they have reported that the position of the apical constriction, apical foramen and the cemento-dentinal junction varies across the tooth types. These anatomical apical landmarks are considered extension limits for root canal instrumentation and filling. Achieving an optimum working length is thought essential for successful root canal treatment, so adopting any of these landmarks is associated with certain risks and benefits. The variability in the position of the apical constriction and apical foramen, for example, complicates their clinical detection, while the cemento-dentinal junction is a histological landmark that cannot be detected clinically. The radiographic apex does not always coincide with the anatomic apex of the tooth. The pre-operative status of the pulp must be considered while obtaining the working length. Most prognostic studies agree that extending the root filling to within 2-3 mm of the radiographic apex is associated with favorable treatment outcomes.
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36,781
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CASE REPORTS
Bio-reconstruction of root canal using dentin post
Navin Mishra, Isha Narang
May-August 2013, 3(2):87-89
DOI
:10.4103/1658-5984.118158
This case report describes the successful management of complicated crown fracture of left maxillary central incisor (#21) of 23-year-old male using dentin (biological) post made from human tooth. Endodontic treatment was initiated and sectional obturation was done using ProTaper gutta-percha and AH Plus sealer. The coronal space was modified to receive a human dentin post. A two-step procedure comprising direct and indirect technique of post fabrication was done to achieve the accuracy of biological post. A maxillary cuspid from an institutional tooth bank was taken, sectioned mesiodistally using a diamond disc simulating the length and thickness of mock post. Dentin post was first verified on the plaster model and then cemented in tooth #21. Core build up was done with composite and porcelain fused to metal (PFM) crown was luted. On the follow-up visits, patient was asymptomatic and radiographic evaluation revealed normal periradicular architecture. Biological posts may be good alternatives to conventional post systems as they preserve internal dentin walls, provide excellent adhesion, and resilience similar to natural tooth structure.
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3
4,018
742
Crown discoloration due to the use of triple antibiotic paste as an endodontic intra-canal medicament
Manuel Sebastian Thomas
January-April 2014, 4(1):32-35
DOI
:10.4103/1658-5984.127985
The use of triple antibiotic paste (TAP) (mixture of ciprofloxacin, metronidazole and minocycline) as an intra-canal medicament have shown very encouraging results particularly in non-vital immature teeth associated with periradicular lesions. Hence, before opting for a surgical approach, a non-surgical approach can be thought of with extended use of TAP as intra-canal medicament, especially in patients who are phobic to invasive procedures. On the flip side, the use of TAP has few side-effects, of which one is the discoloration it can cause due to the presence of minocycline in the mix. The purpose of this article is to presents a case of coronal discoloration due to the use of TAP as an endodontic intra-canal medicament.
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3
12,684
1,374
ORIGINAL ARTICLES
Antibacterial efficacy of Melaleuca alternifolia (Tea tree oil), Curcuma longa (Turmeric), 2% chlorhexidine, and 5% sodium hypochlorite against Enterococcus faecalis: An in vitro study
Dakshita Joy Sinha, Agrima Vasudeva, Natasha Jaiswal, Paridhi Garg, Shashi Prabha Tyagi, Jagvir Singh
September-December 2015, 5(3):182-186
DOI
:10.4103/1658-5984.163623
Aim:
To evaluate and compare the antibacterial efficacy of
Melaleuca alternifolia
(tea tree oil), Curcumalonga (turmeric), 2% chlorhexidine (CHX), and 5% sodium hypochlorite (NaOCl) against
Enterococcus faecalis.
Materials and Methods:
Agar plates were prepared using tryptone soya agar. Cultures of
E. faecalis
were grown in tryptone soya broth. Agar well diffusion method was performed and the plates were incubated at 37C for 24 h. The zones of inhibition were recorded. The readings were subjected to statistical analysis using analysis of variance (ANOVA) and Tukey's post hoc test.
P
-value was considered significant at
P
< 0.05.
Results:
Maximum antibacterial efficacy was exhibited by 2% CHX, followed by 5% NaOCl and C. longa with no statistically significant difference between them. It was followed by M. alternifolia (Tea tree oil). Ethanol and saline showed the least antibacterial action.
Conclusion:
According to the results of this study,
C. longa
and
M. alternifolia
can be used as an alternative root canal irrigant, although long-term in vivo studies are warranted.
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3
4,128
595
“Comparative evaluation of dentinal penetration of three different endodontic sealers with and without smear layer removal” - Scanning electron microscopic study
KR Sonu, TN Girish, KC Ponnappa, KV Kishan, PK Thameem
January-April 2016, 6(1):16-20
DOI
:10.4103/1658-5984.171996
Aim and Objectives:
The aim of this study was to test the dentinal tubule penetration of mineral trioxide aggregate (MTA) Fillapex (Angelus, Londria, PR, Brazil), GuttaFlow
®
2 sealer (Coltene/Whaledent) with standard sealer AH Plus (Dentsply Detrey, Konstanz, Germany) in instrumented root canals obturated by using cold lateral compaction techniques in either the presence or absence of the smear layer.
Materials and Methods:
Sixty extracted human mandibular premolars with a single root canal were used for this study. All teeth were prepared using F2 Pro Taper nickel–titanium rotary system. Teeth were randomly divided into three groups of 20 teeth each. GuttaFlow 2, MTA Fillapex and AH Plus were used as sealers in Group I, Group II, and Group III, respectively. Groups were divided into two subgroups in which either the smear layer was removed or retained. Removal of smear layer was achieved by continuous irrigation with 5.25% sodium hypochlorite and 2 ml of 17% ethylenediaminetetraacetic acid. The canals were dried and obturated using respective sealers by cold lateral compaction techniques. After storage at 37°C and 100% humidity for 14 days, the roots were then split into two halves using chisel and mallet. On each section, sealer penetration in the dentinal tubules at cervical, middle, and apical levels was examined by using scanning electron microscope.
Results:
Sealer penetration into the dentinal tubules was noticed in all groups. AH plus showed deeper penetration, followed by MTA Fillapex and GuttaFlow® 2. The penetration depth of the sealers at the cervical and middle third of root was significantly more than those at apical third.
Conclusions:
Within the parameters of the present study it was concluded, with the removal of smear layer AH plus sealer showed deeper penetration into the dentinal tubules at cervical and middle third of root compared with apical third.
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3
4,749
890
Gutta-percha disinfection: A knowledge, attitude, and practice study among endodontic postgraduate students in India
Venugopal Panuganti, VJ Vivek, CM Jayashankara, S Anilkumar, SA Girish, Jyothi Kashi Nanjundasetty
September-December 2016, 6(3):127-130
DOI
:10.4103/1658-5984.189353
Aim:
(1) To evaluate knowledge, attitude, and practices about methods of gutta-percha disinfection by endodontic postgraduate students in India. (2) To check the sterility of two commercially available, freshly, and previously opened gutta-percha box which was exposed to the clinical environment.
Materials and Methods:
Data were collected in a prescribed format from 400 endodontic postgraduate students. The questionnaire was designed to evaluate their knowledge about standard sterilization protocols of gutta-percha points, attitude toward the utility of guidelines/protocols of sterilization in the success of root canal treatment and practice of these sterilization protocols. For microbial assay, 10 cones of freshly opened gutta-percha 6% taper of size 25 from diadent (Diadent Group International, Korea) Group A and Dentsply (Dentsply Maillefer, Switzerland) Group B. Cones from the same companies which was kept in chair side working environment during obturation were selected as Group C and Group D, respectively, for aerobic and anaerobic culturing.
Results:
Among the respondents 75% did not practice any disinfection protocols. Only 25% followed the disinfection of gutta-percha cones. In anaerobic culture Group B turned out to be positive in all samples, all other groups were negative.
Conclusions:
The endodontic postgraduate students possess adequate knowledge on disinfecting gutta-percha cones, but regular practice of this is uncommon. Microbial assay showed that even freshly opened gutta-percha cones might be contaminated.
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3
5,009
601
An
in vitro
comparative antibacterial study of different concentrations of green tea extracts and 2% chlorhexidine on
Enterococcus faecalis
Leena P Martina, Ambrose Vedamanickam Rajesh Ebenezar, Mohamed Fayas Ghani, Ashwin Narayanan, Meenakshi Sundaram, Ajit George Mohan
September-December 2013, 3(3):120-124
DOI
:10.4103/1658-5984.121504
Aim
: The purpose of this study was to comparatively evaluate the antibacterial property of various concentrations of green tea extracts and 2% chlorhexidine (CHX) against
Enterococcus faecalis 0(E. faecalis
)
in vitro
.
Materials and Methods:
The activity of green tea extract and CHX on
E. faecalis
was measured on agar plates using the agar diffusion method. In addition, the tube dilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration of green tea extract against E. faecalis.
Results:
The MIC of green tea extract was found to be 3.5%. The zone of inhibition was found to be concentration dependent. The 2.5% concentration of green tea extract showed 20 mm zone of inhibition, while the 3% concentration demonstrated a 30 mm zone of inhibition. Two percent CHX showed 30 mm zone of inhibition.
Conclusion:
Three percent concentration of green tea extract showed an antibacterial activity equivalent to 2% CHX against E. faecalis.
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3
4,728
956
Evaluation of calcium hydroxide removal using EndoActivator system: An
in vitro
study
Saad Al-Garni, Saad Al-Shahrani, Saad Al-Nazhan, Nassr Al-Maflehi
January-April 2014, 4(1):13-17
DOI
:10.4103/1658-5984.127981
Aim:
The aim of this study was to compare the calcium hydroxide (Ca(OH)
2
) removal efficacy of the EndoActivator system with that of conventional irrigation with a syringe and hand file manipulation.
Materials and Methods:
A total of 44 extracted human single-rooted mandibular premolars were used. The root canal systems of all teeth were cleaned and shaped, then filled with Ca(OH)
2
paste. The teeth were divided into two groups: Ca(OH)
2
was removed using ethylenediaminetetraacetic acid (EDTA) and sodium hypochlorite (NaOCl) with hand filing and irrigation (Group 1) or the EndoActivator system (Group 2). Each tooth was split into two halves and examined under a scanning electron microscope.
Results:
Ca(OH)
2
particles were completely removed at the coronal level in both experimental groups. More Ca(OH)
2
particles were present in the apical third than in the middle-third in both groups. No significant interaction was observed between the two experimental groups or among the three examined levels (
P
> 0.05).
Conclusion:
Agitation with NaOCl and EDTA improved Ca(OH)
2
removal only in the coronal third of the root canal. Use of the EndoActivator system did not improve the efficacy of Ca(OH)
2
removal in the middle and apical thirds.
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3
4,240
740
Incidence of two canals in extracted mandibular incisors teeth of Saudi Arabian samples
Khalid S Al-Fouzan, Abdulatif AlManee, Jilan Jan, Mansour Al-Rejaie
May-August 2012, 2(2):65-69
DOI
:10.4103/1658-5984.108151
Aim:
The purpose of this
in vitro
study is to provide clinical data on the presence of the second canal in mandibular incisor teeth of Saudi Arabian Samples.
Materials and Methods:
Eighty extracted human mandibular incisors were collected from Saudi patients. The teeth were accessed by small round bur then placed in 5.25% sodium hypochlorite for 24 hours to dissolve the organic debris. Indian ink was injected inside the root canal systems under negative pressure. The teeth were decalcified in 5% nitric acid for three days then dehydrated in different concentrations of ethyl alcohol. Following the decalcification process, the teeth were cleared in methyl salicylate and evaluated according to Vertucci's classification.
Result:
Fifty six of both mandibular central and lateral incisor teeth (70%) had type I canal configuration (one main canal and one main apical foramen), while the remaining 30% of the sample (24 teeth) had a type III canal configuration (two separate canals and merged into one canal before exiting the tooth through single apical foramen).
Conclusion
: The incidence of two canals in mandibular incisor teeth is about one third of the examined Saudi Arabian samples with no difference between the centrals and laterals. The clinician should deal with these teeth as if they have two canals unless it is proved otherwise.
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CASE REPORTS
Managing sub-gingival fracture by multi-disciplinary approach: Endodontics-forced orthodontic extrusion and prosthetic rehabilitation
Rakesh Mittal, Sumit Gupta, Anchal Singla, Anshu Gupta
May-August 2013, 3(2):82-86
DOI
:10.4103/1658-5984.118157
Traumatized anterior teeth with sub-gingival crown fractures are a challenge to treat. The management of sub-gingival fractures includes exposing the cervical margin followed by appropriate coronal restoration. The treatment modalities, which involve exposing the cervical margin, are surgical crown lengthening and orthodontic extrusion. This paper reports a case of fractured maxillary anterior tooth at the sub-gingival level that was managed by forced orthodontic extrusion after endodontic treatment followed by esthetic rehabilitation, a much forgotten technique not utilized routinely yet conservative and cost-effective.
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2
11,611
1,374
Endodontic management of a supernumerary tooth fused to the maxillary permanent lateral Incisor
Avinash A Patil, Sanjana A Patil, Preeti K Dodwad
January-April 2014, 4(1):28-31
DOI
:10.4103/1658-5984.127984
The aim of this case report is to present the endodontic management of a supernumerary tooth fused to the maxillary left lateral incisor. Such anomalies pose a challenge even to the most experienced clinician in treating these teeth. This report described a case of 21-year-old male patient with a classic case of complete fusion of maxillary left lateral incisor and a supernumerary tooth. A modified access preparation was made and two separate root canal orifices were identified. Both root canal systems were prepared separately and obturated efficiently until accepted lengths. This reported case highlights the importance of clinical and radiographic correlation in arriving to a definitive diagnosis.
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2
3,283
389
ORIGINAL ARTICLES
Comparative evaluation of the cytotoxicity of 5.25% sodium hypochlorite, 2% chlorhexidine and mixture of a tetracycline isomer, an acid and a detergent on human red blood corpuscles: An
in-vitro
study
Krishna Prasad Shetty, Sarvepalli Venkata Satish, Krishnarao Kilaru, Kalyana Chakravarthi Ponangi, Vijay Reddy Venumuddala, P Ratnakar
January-April 2014, 4(1):1-6
DOI
:10.4103/1658-5984.127979
Aim:
The aim of this study was to analyze the cytotoxicity of various volumes of 5.25% of sodium hypochlorite, 2% of chlorhexidine gluconate and mixture of a tetracycline isomer, an acid and a detergent (MTAD) by checking for hemolysis of human red blood corpuscles.
Materials
and
Methods:
A total volume of 100 μl of diluted red blood corpuscles obtained through centrifugation was added to three irrigating solutions (5.25% of sodium hypochlorite, 2% of chlorhexidine and MTAD) of six test tubes each. Individual irrigants per group were added in increasing volume starting from the second test tube keeping the first one as the control. After incubation for 3 min hemoglobin content was measured using an automated hemoanalyzer data was analyzed using one sample
t
-test.
Results:
Sodium hypochlorite is the most cytotoxic solution followed by MTAD and chlorhexidine.
Conclusions:
This study suggests that the three irrigating solutions do cause detrimental effects on the diluted red blood corpuscles. A great deal of care should therefore be exercised when using 5.25% sodium hypochlorite during endodontic irrigation.
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2
4,532
678
Biocompatibility assessment of modified Portland cement in comparison with MTA
®
:
In vivo
and
in vitro
studies
I Khalil, J Isaac, C Chaccar, JM Sautier, A Berdal, N Naaman, A Naaman
January-April 2012, 2(1):6-13
DOI
:10.4103/1658-5984.104415
Aim:
The aim of our study is to elaborate a new cement based on Portland cement (PC), Modified Portland Cement (MPC) with modified chemical and physical properties that allow easier clinical manipulation and faster setting time than MTA
®
and then to evaluate its cytotoxicity
in vitro
and its biocompatibility
in vivo
in comparison with MTA
®
.
Materials and Methods:
Elaboration of MPC: Portland cement powder slenderly grinded to homogenize the particles, mixed with a radiopaque element and a setting time accelerator. A comparative
in vitro
study (MTS test) of the toxic effect of MTA
®
and MPC with culture isolated from the calvaria of 18-day-old fetal Swiss OF1 mice are done. A comparative
in vivo
study of the biocompatibility of MTA
®
and MPC: Under general anaesthesia, three holes (2.5 mm) were made in both the left and right femurs of six White New Zealand rabbits. In the first hole MPC is placed, in the second MTA
®
and the third one is left empty (negative control group). Three weeks after implantation, two rabbits are sacrificed, then two other rabbits over six weeks and the last two after twelve weeks. The neck of the femur is trimmed and prepared for undecalcified histological studies. Mann-Whitney test was used to analyze the results.
Results:
The cell viability test according to the morphological observations suggested the biocompatibility of the two biomaterials tested. The
in vivo
test showed similar biocompatibility between MTA
®
and MPC. Bone healing and minimal inflammatory response adjacent to MTA
®
and MPC implants were observed at all experimental periods (3, 6 and 12 weeks), suggesting that both materials are well tolerated.
Conclusion:
This pilot comparative study of MTA
®
and MPC showed no or very limited toxic effects of both cements
in vitro
and similar biocompatibility
in vivo.
However, additional
in vivo
and clinical studies should be done on MPC before it can be introduced in our clinical practice.
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2
4,863
588
Radiographic evaluation of the quality of root canal obturation of single-matched cone Gutta-percha root canal filling versus hot lateral technique
Randa Suleiman Obeidat, Hassan Abdallah
May-August 2014, 4(2):58-63
DOI
:10.4103/1658-5984.132717
Aim:
The aim of this study is to evaluate radiographically the quality of root canal filling in mesiodistal and buccolingual view when comparing matched cone condensation and warm lateral Gutta-percha condensation using system B heating instrument in a low-heat warm lateral condensation technique
in
0
vitro
.
Materials
and Methods:
A total of 40 mandibular premolars with straight single canals were divided into two groups with 20 each. The root canals were shaped by hand file and Revo-S rotary files to size (25, 0.06) at the end point, then they filled by Gutta-percha cone and meta-seal sealer. In group A, a single matched cone technique was used to fill the root canals. In group B, a hot lateral condensation using system B instrument at 101°C was performed.
Result:
The result of this study showed no significant difference in density of Gutta-percha fill in apical and coronal two-third when comparing matched cone root canal filling and hot lateral technique (P > 0.05). The only significant difference (P < 0.05) was in matched cone between buccolingual and mesiodistal view in the coronal two-third.
Conclusion:
Within the limitation of this study, single matched cone technique has a good density in the apical one-third as that of the hot lateral technique so it may be used for filling narrow canals. In the coronal two-third of the root canal, single matched cone technique showed inferior density of root canal filling which can be improved by using accessory cones Gutta-percha in wide canal.
[ABSTRACT]
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[CITATIONS]
2
5,026
646
Volumetric analysis of root canals obturated with cold lateral condensation, single-cone and thermoplasticized gutta-percha techniques using spiral computed tomography: An
in vitro
study
Shanol Anusha Crasta, Jyothi Kashi Nanjundasetty, Venugopal Panuganti, Jayashankara Chatra Marigowda, Sharath Kumar, Anil Kumar
May-August 2014, 4(2):64-69
DOI
:10.4103/1658-5984.132720
Aim:
To evaluate and compare the volume percentage of root canals obturated with gutta percha (POV), with various techniques using spiral computed tomography (SCT).
Materials and Methods:
Forty-five mandibular first premolar teeth were instrumented using Race files and randomly divided into three groups of 15 teeth each (
n
= 15). The volume of root canal space was measured using SCT and the root canals were obturated as follows: Group 1-lateral condensation, Group 2-single-cone obturation and Group 3-thermoplasticized gutta-percha technique. The filled volume of root canals was measured using SCT and POV was calculated in total and at apical, middle and coronal third individually. The data was statistically analyzed using one-way ANOVA and Tukeys
post hoc
multiple comparison tests.
Results:
An intergroup comparison of the mean value of POV showed a statistically significant difference (
P
< 0.05) in the middle third and in total when group 1 was compared to group 2.
Conclusion:
All the groups showed 100% POV at the apical third. Group 2 showed least POV at the middle third of the root canal.
[ABSTRACT]
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[CITATIONS]
2
5,455
704
REVIEW ARTICLE
The accuracy of Root ZX electronic apex locator
Osama S Alothmani
September-December 2012, 2(3):115-130
DOI
:10.4103/1658-5984.112702
The aim of this review was to evaluate studies assessing the accuracy of Root ZX when used for working length determination in permanent teeth and to identify factors affecting the device's precision. An electronic search for articles published in English language since 1994 was conducted on the Medline via Ovid interface. All issues of the International Endodontic Journal, Journal of Endodontics and Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology since 1994 were manually searched. The reference lists of review articles were cross-referenced to identify any potential publications. A total of 76 publications fulfilled the inclusion criteria. The studies varied in their methodologies, and most of them did not adhere to the manufacturer's recommendations while operating the device. The Root ZX failed to consistently detect the apical constriction or the apical foramen. Nonetheless, it mostly allowed file tip placement in the area bounded by these two landmarks, especially when the 0.5 mark of its digital meter was adopted. Tooth-related factors potentially influencing the precision of Root ZX included pre-operative pulp status, tooth type, position of the apical foramen, canal obliteration, and the size of apical diameter. Operative factors including coronal pre-flaring, presence or absence of irrigants, file size, file alloy, and mode of file operation could also influence the performance of Root ZX. In conclusion, adopting the 0.5 mark of the digital meter of the Root ZX reduces chances of violating the apical foramen. Further, factors influencing the precision of Root ZX must be considered while operating it.
[ABSTRACT]
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2
7,360
4,522
Radiographic assessment of endodontic working length
Osama S Alothmani, Lara T Friedlander, Nicholas P Chandler
May-August 2013, 3(2):57-64
DOI
:10.4103/1658-5984.118145
The use of radiographs for working length determination is usual practice in endodontics. Exposing radiographs following the principles of the paralleling technique allows more accurate length determination compared to the bisecting-angle method. However, it has been reported that up to 28.5% of cases can have the file tip extending beyond the confines of the root canals despite an acceptable radiographic appearance. The accuracy of radiographic working length determination could be affected by the location of the apical foramen, tooth type, canal curvature and superimposition of surrounding structures. Variations among observers by virtue of training and experience may also influence the accuracy of the procedure. The interpretation of radiographs could be affected by film speed and viewing conditions, with the superiority of digital imaging over conventional radiography for working length determination remaining debatable. The combination of several methods is recommended for acquiring the most accurate working length.
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13,505
1,435
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Online since 05 July, 2012