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LETTER TO EDITOR
Year : 2022  |  Volume : 12  |  Issue : 3  |  Page : 338

Scanning electron microscope - The right tool for smear layer analysis?


Department of Conservative Dentistry and Endodontics, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India

Date of Submission09-Jan-2022
Date of Decision02-Mar-2022
Date of Acceptance02-Mar-2022
Date of Web Publication1-Sep-2022

Correspondence Address:
Dr. Nidambur Vasudev Ballal
Department of Conservative Dentistry and Endodontics, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Manipal - 576 104, Karnataka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/sej.sej_10_22

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How to cite this article:
Ballal NV. Scanning electron microscope - The right tool for smear layer analysis?. Saudi Endod J 2022;12:338

How to cite this URL:
Ballal NV. Scanning electron microscope - The right tool for smear layer analysis?. Saudi Endod J [serial online] 2022 [cited 2022 Oct 5];12:338. Available from: https://www.saudiendodj.com/text.asp?2022/12/3/338/354823

Dear Sir,

I read with great interest the article entitled, “Effect of sonic irrigation activation at different frequencies in smear layer removal; an in vitro experimental study” by Aalmohamed et al., which has been published in your esteemed journal.[1] Even though the study was well performed, I would like to share my thoughts on the technique used to analyze the presence or absence of smear layer. The current study used conventional scanning electron microscope (SEM) for smear layer analysis. However, SEM model is not a sound, reproducible, and valid one. Ideally, a longitudinal observation of the canal using micro-computed tomography is regarded a fundamental requirement to study the smear layer removal procedures. Thus, the SEM analysis for smear layer removal which does not allow this longitudinal observation of dentinal morphology compromises the credibility of the results.[2] Further, in the current study, the samples were not anatomically matched. Knowing that the instruments used to shape the canals touch only 50% of the canal walls,[3],[4] what was the guarantee that the areas imaged in SEM had a smear layer to begin with? To mitigate this problem, a split tooth model using e-SEM would have been more appropriate.[5]

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Conflicts of interest

There are no conflicts of interest.

 
  References Top

1.
Aalmohamed E, Ahmed F, Alfardan L, El Abed R, Khamis AH, Jamal M. Effect of sonic irrigation activation at different frequencies in smear layer removal; An in vitro experimental study. Saudi Endod J 2022;12:106-13.  Back to cited text no. 1
  [Full text]  
2.
De-Deus G, Reis C, Paciornik S. Critical appraisal of published smear layer-removal studies: Methodological issues. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011;112:531-43.  Back to cited text no. 2
    
3.
Paqué F, Zehnder M, De-Deus G. Microtomography-based comparison of reciprocating single-file F2 ProTaper technique versus rotary full sequence. J Endod 2011;37:1394-7.  Back to cited text no. 3
    
4.
Peters OA, Arias A, Paqué F. A micro-computed tomographic assessment of root canal preparation with a novel instrument, TRUShape, in mesial roots of mandibular molars. J Endod 2015;41:1545-50.  Back to cited text no. 4
    
5.
Schmidt TF, Teixeira CS, Felippe MC, Felippe WT, Pashley DH, Bortoluzzi EA. Effect of ultrasonic activation of irrigants on smear layer removal. J Endod 2015;41:1359-63.  Back to cited text no. 5
    




 

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