Home Print this page Email this page Users Online: 180
Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
ORIGINAL ARTICLE
Year : 2015  |  Volume : 5  |  Issue : 2  |  Page : 125-128

Effect of sonic agitation, manual dynamic agitation on removal of Enterococcus faecalis biofilm


1 Department of Conservative and Endodontics, Vananchan dental college. Farathiya, Hoor, Garwah, Jharkhand, India
2 Department of Conservative Dentistry and Endodontics, Sree Siddhartha Dental College and Hospital, Karnataka, India

Correspondence Address:
Rajshekhar Chatterjee
C/O: Senior lecturer, Department of Conservative and Endodontics. Vananchan Dental College. Farathiya, Hoor, Garwah. Jharkhand - 822 114
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1658-5984.155451

Rights and Permissions

Objectives: The aim of the study was to compare manual dynamic agitation with sonic agitation on removal of intra radicular Enterococcus faecalis (E. faecalis) biofilm. Material and Methods: Extracted mandibular premolars for orthodontic purpose were sectioned at cervical level and divided into three groups (n = 30). The root canals were instrumented using Protaper rotary instruments up to apical file F4. Roots were sterilized and E. faecalis bacteria were incubated within their root canal space for four weeks. Confirmation of biofilm was done using scanning electron microscopy and Gram staining. All groups were irrigated with side vented needle by using three percent sodium hypochlorite (NaOCl) for 60 seconds. Two experimental groups were agitated with manual dynamic agitation (with master gutta-percha cone) and sonic agitation (EndoActivator). Remaining bacteria were collected using sterile paper point, which were incubated inside brain-heart infusion (BHI) broth to check turbidity. The turbid broth was streaked on blood agar plate for colony counts. Result: Both experimental groups showed highly significant difference in their mean colony count when compared with control group; with P < 0.001. Conclusion: Passive sonic agitation with EndoActivator has proven to be the best irrigating system followed by manual dynamic agitation and conventional needle irrigation.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2716    
    Printed43    
    Emailed0    
    PDF Downloaded461    
    Comments [Add]    
    Cited by others 1    

Recommend this journal