|LETTER TO EDITOR
|Year : 2015 | Volume
| Issue : 1 | Page : 65-66
Department of Conservative Dentistry and Endodontics, Kothiwal Dental College and Research Centre, Moradabad, Uttar Pradesh, India
|Date of Web Publication||12-Jan-2015|
Department of Conservative Dentistry and Endodontics, Kothiwal Dental College and Research Centre, Moradabad - 244 001, Uttar Pradesh
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Garg P. Author's Reply. Saudi Endod J 2015;5:65-6
I am glad that you read my article with so much intricacy and took out time to cite these valid questions.
Sir, it is mentioned in the article that the samples were sterilized by ultraviolet radiation with a dosage 300 kJ/cm 2 for 10 minutes in the laminar airflow. However, all the specimens were also sterilized in an autoclave for two cycles. The first cycle at 121°C and the second with the specimens immersed in 1 mL of brain heart infusion (BHI) broth in individual microcentrifuge tubes before starting. 
As far as the dentinal tubule penetration is concerned, it has been studied in further studies. This was a preliminary study which was conducted to ascertain which herbal irrigants are efficacious against E. faecalis. I have conducted further studies on dentinal tubule disinfection with the irrigants which gave the best results in this study, which should be published soon.
According to Zehnder,  chlorhexidine cannot be advocated as the main irrigant in standard endodontic cases, because chlorhexidine is unable to dissolve necrotic tissue remnants. Thus, it was not included in this study as it was to ascertain the antimicrobial efficacy. However, it has been included in the further studies on dentinal tubule disinfection.
The purity of the culture was confirmed by gram staining, catalase production test, and colony morphology on blood agar infusion broth agar. 
For antimicrobial efficacy studies, paper point method has been suggested.  Dentinal shavings has been suggested for dentinal tubule disinfection studies, thus this study model has been used in the further studies. 
| References|| |
Krithikadatta J, Indira R, Dorothykalyani AL. Disinfection of dentinal tubules with 2% chlorhexidine, 2% metronidazole, bioactive glass when compared with calcium hydroxide as intracanal medicaments. J Endod 2007;33:1473-6.
Zehnder M. Root canal irrigants. J Endod 2006;32:389-98.
Gomes BP, Souza SF, Ferraz CC, Teixeira FB, Zaia AA, Valdrighi L, et al
. Effectiveness of 2% chlorhexidine gel and calcium hydroxide against Enterococcus faecalis
in bovine root dentine in vitro
. Int Endod J 2003;36:267-75.